The effect of genes expression connected with glycometabolism and fatty acid metabolism in the brain of the high-intensity endurance training rat was studied using cDNA microarray. Rats were divided into the quiet group and the strong intensity endurance training group at random. The both groups freely ingest and drink. The quiet group of rats was bred quietly. The group with high-intensity endurance training was put to death after training for 7 weeks. Brains were taken quickly to extract mRNA for reverse transcription cDNA for detection. There were the 4 and 8 strips of genes connected with glycometabolism and fatty acid metabolism expressed in rats′ brain，respectively, and all were down-regulation. The genetic expression product related to glycometabolism were LDH3, UDP-glucuronosyltransferase, Glucose-6-phosphatase (G6pc), phosphoglyceromutase, and fatty acid metabolism were carboxyl ester lipase, paraoxonase, stearyl-CoA desaturase, sulfotransferase, Apolipoprotein A(-I and -V), arylacetamide deacetylase and lysophosphatidic acid acyltransferase-delta, separately.The genes connected with glycometabolism and fatty acid metabolism were screened in the brain of the high-intensity endurance training rat and all down-regulation. The results revealed that the high-intensity endurance training could restrain glycometabolism and fatty acid metabolism in the brain of rat, which was one of the biology mechanism of the center fatigue.

high-intensity endurance training; gene expression; glycometabolism；fatty acid metabolism；brain tissue