The inhibition effect of two different momordica charantia polysaccharides on the proliferation of human leukemia cell in vitro
LI Zhe-tao1, ZHANG Jing2*, XIE Jia2, SUN Run-guang1
(1 Laboratory of Biophysics and Biomedical Technology,College of Physics and Information Technology,Shaanxi Normal University;2 Natural Product Chemistry Laboratory, College of Food Engineering and Nutrition Science,Shaanxi Normal University, Xi′an 710062, Shaanxi, China)
Abstract:
The crude polysaccharides were extracted from momordica charantia using hot water. Then they were isolated and purified by DEAE-52-cellulose anion exchange chromatography. After eluted by double distilled water and 0.1 mol/L NaCl respectively, the W-MCP and S-MCP were obtained. The in vitro inhibition activity of W-MCP and S-MCP on the proliferation of human leukemia cell line K562 were measured by using MTT assay. The in vitro antioxidant activity including O2-· and DPPH free radical scavenging effect and lipid peroxidation inhibition effect of W-MCP and S-MCP were determined. Our results showed that the inhibition rate on the proliferation of human leukemia cell line K562 of W-MCP and S-MCP increased with time. The inhibition rate tended to be stable at 48h, and the largest inhibition rate of W-MCP and S-MCP reached 30.46% and 28.61% at the concentration of 400μg/mL at 72h. Both W-MCP and S-MCP have high scavenging rate on the O2-·, reaching 58.52% and 44.71%. The DPPH scavenging rate of W-MCP and S-MCP decreased with the concentration, and the highest scavenging rate were 19.01% and 30.11%, respectively. Lipid peroxidation inhibition effect of W-MCP and S-MCP was low. Therefore, balsam pear polysaccharides had certain antioxidant activity and inhibition effect on cancer cell proliferation.
KeyWords:
water elution balsam pear polysaccharide; salt elution balsam pear polysaccharide; antitumor; antioxidant
