Expression pattern of farnesyl diphosphate synthase(SmFPPS1) from Salvia Miltiorrhiza Bunge
ZHOU Lu1, HUA Wen-ping1,2, YANG Ying1, WANG Zhe-zhi1, LI Cui-qin1*
(1 Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, Shaanxi Normal University, Xi′an 710062,Shaanxi,China; 2 Shaanxi Institute of Education, Department of Life Scienceand Technology, Xi′an 710100, Shaanxi, China)
Abstract:
Tanshinones are the major active components of Danshen, which are derived from terpenoids, and farnesyl diphosphate synthase (FPPS) is a key enzyme in terpenoids biosynthetic pathway. A novel FPPS(SmFPPS1, HQ687768) in Salvia miltiorrhiza was cloned and characterized. Bioinformatics analysis showed that SmFPPS1 locates in cytoplasm, and it contains a prenyltransferase domain and two Asp-rich characteristic motifs. The functional complementation tests showed that SmFPPS1 didn′t exhibit activity of GGPPS. Quantitative real-time PCR analysis revealed that the gene expressed constitutively in different development periods, mainly in flowers, and the expression could be induced by methyl jasmonate (MeJA) and fungal elicitor signal.
KeyWords:
Salvia miltiorrhiza; farnesyl diphosphate synthase; expression pattern
