Oxidative damage of H-22 tumor-bearing mice by Chlorin e6 in sonodynamic therapy
CHEN Yan, WANG Lu, SHI Hai-tao, WANG Pan*, LIU Quan-hong
(College of Life Science, Shaanxi Normal University, Xi′an 710062, Shaanxi，China)
Abstract:
The synergistically killing effect of Chlorine e6 combining with ultrasound on Ehrlich ascites tumor bearing mice was determined. Enzymatic chemical method was used to measure the lipid peroxidation levels and activities of key antioxidant enzymes (ie, superoxide dismutase, glutathione peroxidase, catalase activities and MDA level) in Ehrlich ascites tumor after treatment. The results indicated that Chlorine e6 combining with ultrasound could inhibit the growth of Ehrlich ascites tumor tissue.Superoxide dismutase, glutathione peroxidase and catalase activities decreased and MDA level increased significantly in the treated tumor tissue. The changes in antioxidant enzyme activities and MDA level suggest that oxidative damage may play an important role in the anti-tumor effect in Ehrlich ascites tumor bearing mice treated by Chlorine e6 combining with ultrasound.
KeyWords:
Chlorin e6; sonodynamic therapy; H-22 tumor-bearing; antioxidative enzymes
