ZHENG Peng1, HUA Wen-ping1,2, WANG Zhe-zhi1*
(1 Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, College of Life Science, Shaanxi Normal University, Xi′an 710062, Shaanxi, China; 2. Shaanxi Xueqian Normal University, Department of Life Sciences and Technology, Xi′an 710100, Shaanxi, China)
Abstract:
Gentiopicroside, a secoiridoid glycoside, is one of the main pharmacological compounds of traditional Chinese medicine Gentiana. In this study, two Gentiana macrophylla HMGR genes were cloned based on our laboratory′s high-throughput sequencing, and bioinformatics analysis was carried out.The results showed that two HMGR genes both contain the completed ORF, and their encoded proteins with two transmembrane domains and four HMGR protein-specific conserved active sites, high consistency with the other plant in HMGR protein sequences and high structure, but GmHMGR1 and GmHMGR2 locate respectively in the cell plasma membrane and mitochondria, Quantitative PCR analysis showed that GmHMGR1 has high expression levels in the roots and flowers in G. macrophylla, while GmHMGR2 mainly expresses in leaves.
KeyWords:
Gentiana macrophylla; 3-hydroxy-3-methyl-glutaryl-CoA reductase(HMGR); high-throughput sequencing; sequence analysis
