XING Rui-huan, MOU Mao-sen, GAO Xiao-jie, NIE Hong-juan, YANG Min-min,XU Zhu, XIONG Yan, WU Qin-feng, CHEN Yi-wen, SONG Ming, HOU Ying-chun*
(College of Life Science, Shaanxi Normal University, Xi′an 710062, Shaanxi, China)
Abstract:
To study the roles played by ANXA2 during the development of colorectal carcinoma, and the relationship between the structure and function of ANXA2, ANXA2 knockout recombinant was constructed and transfected into Caco2 cell line. After a series of selecting step, ANXA2-/-Caco2 cell line was successfully generated. By the designated PCR and Western blot analysis, it was confirmed that the wild type ANXA2 expression is knockout completely in this cell line.
KeyWords:
Annexin A2; gene knockout; Caco2 cell
