Atomic force microscopy imaging of different concentration of low-density lipoproteins
XUE Hui-jun1, WANG Jian-qi1, LU Guo-hua1, ZHANG Yang1, JIAO Teng1, SUN Run-guang2*
(1 College of Biomedical Engineering, The Fourth Military Medical University,Xi′an 710032, Shaanxi, China; 2 College of Physics and Information Technology,Shaanxi Normal University, Xi′an 710062,Shaanxi, China)
Abstract:
To study relations of the content of low-density lipoproteins(LDL) with atherosclerosis,the purified LDL from hen egg yolk with concentrations of 5μg/mL, 10μg/mL, 30μg/mL, were observed by atomic force microscopy (AFM).The following results were obtained. Lipoprotein granule at 5μg/mL distributes sparsely, and a few lipoprotein congeries in the system; For lipoprotein solution at 10μg/mL, a clear AFM image of LDL aggregates was found; Because the lipoprotein solution at 30μg/mL was thickness, there were many LDL polymer chains. All results indicated that the degree of LDL assemble was increased with concentration of LDL, which likely causes atherosclerosis.
KeyWords:
low-density lipoproteins; atherosclerosis; atomic force microscopy
