自然科学版
陕西师范大学学报(自然科学版)
生命科学
荔枝草总黄酮的体外抗氧化研究
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师梅梅,杨建雄*,任维
(陕西师范大学 生命科学学院, 陕西 西安 710062)
师梅梅,女,博士研究生,主要研究方向为天然产物的分离鉴定及功能评价.E-mail:shimeimei1982@163.com.* 通信作者:杨建雄,男,教授. E-mail: jxyang@snnu.edu.cn.
摘要:
采用化学体系模拟法体外测定荔枝草总黄酮(TFS)对1,1-二苯基-2-苦苯肼自由基(DPPH·) 和超氧阴离子自由基(O-·2 )的清除能力,总还原力及其对Fe2+诱导的脂质过氧化反应和β-胡萝卜素/亚油酸自氧化体系的抑制作用.结果显示:TFS对DPPH·和O-·2 均有较强的清除能力, IC50值分别为0.079 mg/mL和0.26 mg/mL,有显著的还原力和总抗氧化力,对脂质过氧化有明显的抑制作用.说明荔枝草黄酮可作为天然抗氧化剂开发利用.
关键词:
荔枝草; 黄酮; 抗氧化; 自由基清除
收稿日期:
2010-12-23
中图分类号:
R151. 2
文献标识码:
A
文章编号:
1672-4291(2012)05-0060-04
基金项目:
国家自然科学基金资助项目(20575039).
Doi:
In vitro antioxidant activity of the total flavonoid from Salvia plebeia
SHI Mei-mei, YANG Jian-xiong*, REN Wei
(College of Life Science, Shaanxi Normal University, Xi′an 710062, Shaanxi, China)
Abstract:
The antioxidant activities of total flavonoid from Salvia plebeia (TFS) was evaluated by various in vitro assay systems, such as the β-carotene- linoleic acid model system, reducing power and lipid peroxidation assay, 1, 1-diphenyl-2-picrylhydrazyl (DPPH·) / superoxide (O-·2 ) radical scavenging. TFS showed a significant radical scavenging activity, and the IC50 values for DPPH and superoxide scavenging assay were found to be 0.079 and 0.26 mg/ml, respectively. The reducing power and total antioxidant activity were notable and increasing steadily in a concentration dependent manner. In addition, TFS had effective inhibition of lipid peroxidation. The results indicated that TFS have a potential source of antioxidants of natural origin.
KeyWords:
Salvia plebeia; total flavonoid; antioxidant; free radical-scavenging