自然科学版
陕西师范大学学报(自然科学版)
生命科学
茶多酚提取预处理工艺
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李忠岐, 刘小乔, 高静, 杨建雄*
(陕西师范大学 生命科学学院, 陕西 西安 710062)
李忠岐,男,实验师,硕士研究生,主要研究方向为应用生物化学.E-mail:lzq@snnu.edu.cn.*通信作者: 杨建雄,男,教授.E-mail:jxyang@snnu.edu.cn.
摘要:
以陕西紫阳富硒绿茶为原料,以杂质浸出率和茶多酚损失率为试验指标,在单因素试验的基础上,通过正交试验对茶多酚提取预处理工艺进行优化.结果表明,料液比1∶15(W∶V),25 ℃的水浸渍茶叶20 min,杂质浸出率12.27%,茶多酚损失率3.92%.预处理后的茶叶用85 ℃水浴浸提60 min得茶多酚粗品,其纯度比对照提高了38.50%,咖啡碱含量比对照降低了32.74%.
关键词:
绿茶; 茶多酚; 预处理; 纯度
收稿日期:
2011-11-15
中图分类号:
TS202.3
文献标识码:
A
文章编号:
1672-4291(2012)04-0061-04
基金项目:
国家自然科学基金资助项目(10474061).
Doi:
Study on pretreatment conditions of green-tea for extraction of tea polyphenols
LI Zhong-qi, LIU Xiao-qiao, GAO Jing, YANG Jian-xiong*
(College of Life Science, Shaanxi Normal University, Xi′an 710062, Shaanxi, China)
Abstract:
Using low-end Se-enriched green tea produced by Ziyang county as experimental material, the optimal pretreatment conditions of green tea for extracting tea polyphenols were investigated by single factor and orthogonal test, according to the impurity extraction rate and tea polyphenols loss rate. The experimental results showed that the best ratio of green tea to water, extracting temperature, and extracting time were 1∶15(W∶V), 25 ℃, and 20min with impurity extraction rate of 12.27% and tea polyphenols loss rate of 3.92%. When the pretreated green tea was extracted by water for 60 min at 85 ℃, crude product purity increased by 38.50%, while the content of caffeine reduced by 32.74% compared with the control.
KeyWords:
green tea; tea polyphenols; pretreatment; purity