Preparation of lysozyme based molecularly imprinted polymers and its chromatographic evaluation
HAN Yan-ting, MA Jin-qin, YUE Xuan-feng, ZHANG Jing*
(Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, College of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi′an 710062, Shaanxi, China)
Abstract:
Lysozyme-based molecularly imprinted polymers(Lys-MIPs) were prepared with PGMA/EDMA beads as a carrier material, lysozyme as the template molecule, β-cyclodextrin and acrylamide as the double functional monomers, N,N′-methylene bisacrylamide as the crosslinker. The prepared Lys-MIPs were used as a new HPLC packing material, based on which the related study on its recognition was conducted through the frontier chromatography. The results showed that Lys-MIPs had specific recognition for lysozyme, which helped achieve the separation between lysozyme and three individual competitive proteins, including bovine serum albumin(BSA), ovalbumin(OB) and myoglobin (Myo).
KeyWords:
poly (glycidylmethacrylate-co-ethylenedimethacrylate) (PGMA/EDMA)beads; lysozyme; molecular imprinting; chromatographic separation
