Synthesis and properties of thermo-responsive macroporous PAM-co-PNIPAM microspheres for peetinase immobilization
NA Li-li, YANG Hong*, DENG Hai-juan, LIAO Xian-fu, ZHOU Yan, CHANG Huan， JIN Jing, DING Sheng-gen
(College of Chemistry and Chemical Engineering,Shaanxi Normal University, Xi′an 710062, Shaanxi,China)
Abstract:
Pectinase was immobilized onto the thermo-responsive macroporous polyacrylamide (PAM) microspheres synthesized via an inverse suspension polymerization. The stability of the macroporous PAM supporter with large surface area is not affected by the enzyme even if the amount of enzyme is immobilized up to 322.6 mg/g.The activity of the immobilized enzyme at 50~60 ℃,ant 3.5~5.0(pH) is also stable over 30 days.68% residual activity of the immobilized enzyme was maintained after using 20 batches.Additionally,the porous structure can be changed because PAM-co-DNIPAM supporter is a temperature-depant polymer.Based on this feature,the activity of immobilized pectinase can be significantly adjusted at the lcst of PAM-co-PNIPAM supporter.
KeyWords:
inverse suspension polymerization; poly(N-isopropylacrylamide); thermo-responsive macroporous materal; immobilized pectinase enzyme
