自然科学版
陕西师范大学学报(自然科学版)
化学与材料科学
基于28SrDNA的蝗总科部分种类的分子系统发育研究
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张小静, 郑哲民*, 赵玲, 孙慧敏, 许姝娟, 李鑫
(陕西师范大学 动物研究所,陕西 西安 710062)
张小静,女,硕士研究生,主要研究方向为生物化学.E-mail:xiaojingzhang1982@163.com.*通信作者: 郑哲民,教授,博士研究生导师.E-mail:zhengzhemin@163.com.
摘要:
基于分子系统学方面对蝗总科7科20种昆虫的系统发育进行了探讨.采用PCR 产物直接测序法测定了该20种昆虫的核基因28SrDNA 的部分片段序列,并从 GenBank中下载的摩门螽蟖和变色乌蜢作为外群,采用最大简约法(MP)构建了分子系统树.在获得的504bp序列中, 有340个保守位点,161个变异位点, 45个简约信息位点;G +C 约占69.8%,A+T约占30.2 %.分子系统树表明: 蝗总科7科中槌角蝗科、网翅蝗科和剑角蝗科的分类地位有待进一步的研究,而其他科的分类地位基本与形态学分类一致.
关键词:
蝗总科; 28SrDNA; 分子系统树; 系统发育
收稿日期:
2010-11-15
中图分类号:
Q969.26
文献标识码:
A
文章编号:
1672-4291(2012)02-0067-04
基金项目:
国家自然科学基金资助项目(39391800)
Doi:
Molecular evolution and phylogenetic analysis of some speciesof Arcypteridae based on 28SrDNA gene
ZHANG Xiao-jing, ZHENG Zhe-min, ZHAO Ling, SUN Hui-min, XU Shu-juan, LI Xin
(Institute of Zoology, Shaanxi Normal University, Xi′an, 710062,Shaanxi, China)
Abstract:
Partial species of Acridoidea(20 species, 7 families)were studied in molecular systematics. The partial 28SrDNA gene sequence of 20 species, and two outgroup species, Anabrus simplex and Erianthus versicolor were sampled in this study. The phylogenetic relationships of these species from family level were reconseructed by using Maximum Parsimony (MP) method. There were 340 conserved sites, 161 variable sites and 45 parsimony informative sites, and G+ C was about 69.8% and A + T was only about 30.2 % in the 504 nucleotides of the data. The results showed that taxonomic status of some specie, of Gomphoceridae, Arcypteridae and Acrididae should be researched further,however, taxonomic status of some species of other 4 families are consistent with morphological taxonomy in Acridoidea.
KeyWords:
acridoidea; 28SrDNA; phylogenetic tree; phylogeny