不同品级拟黑多刺蚁（Polyrhachis vicina）脑部雌激素相关受体基因的表达-陕西师范大学学报期刊社网站

陕西师范大学学报（自然科学版）

化学与材料科学

不同品级拟黑多刺蚁（Polyrhachis vicina）脑部雌激素相关受体基因的表达

欧阳霞辉1,2 , 李春鸣1, 魏锁成1 , 侯兰新1, 奚耕思2

(1 西北民族大学生命科学与工程学院，甘肃兰州 730030；2 陕西师范大学生命科学学院，陕西西安 710062)

欧阳霞辉，女，副教授，博士，主要从事昆虫生殖与发育方面的研究. Email:oyxhui316@126.com.

摘要:

采用原位杂交方法对不同品级拟黑多刺蚁（Polyrhachis vicina）脑部雌激素相关受体pvERR mRNA的表达进行了检测.结果表明，pvERR mRNA在不同品级的蕈形体、附叶、视叶、中脑、食道下神经节和球状细胞中都有表达.蚁后蕈形体和球状细胞中pvERR mRNA 的阳性表达最为明显；工蚁中脑pvERR mRNA的阳性反应强度明显高于蚁后和雄蚁；pvERR mRNA在雄蚁视叶中有较强的表达.pvERR mRNA在不同品级的拟黑多刺蚁脑部的表达差异可能与它们各自在蚁群中的功能和行为有关.

关键词：

拟黑多刺蚁；pvERR；原位杂交；昆虫脑部

收稿日期：

2011-03-30

中图分类号：

Q965

文献标识码：

文章编号：

16724291(2012)03006205

基金项目：

国家自然科学基金资助项目（31060350）; 中央高校基本科研业务费专项资金项目（ZYZ2011067）．

Doi:

Expression of estrogen related receptor gene in the brain of different caste of Polyrhachis vicina

OUYANG Xiahui1,2 , LI Chunming1, WEI Suocheng1, HOU Lanxin1, XI Gengsi2

(1 College of Life Science and Engineering, Northwest University for Nationalities, Lanzhou 730030, Gansu, China；2 College of Life Science, Shaanxi Normal University, Xi′an 710062, Shaanxi, China)

Abstract:

The expression of pvERR mRNA in the brain of different caste of Polyrhachis vicina was detected by in situ hybridization method. The results showed that pvERR mRNA has broad expression pattern in the brain of different castes, which can be detected in mushroom body, accessory lobe, optic lobes, deutocerebrum, suboesophageal ganglion and kenyon cells. Strongly positive expression was detected in mushroom body and kenyon cells in brain of queen ant. Compared with queen ant, the expression degree of pvERR mRNA is stronger in the deutocerebrum of worker ant, but other parts are equivalent. Compared with queen and worker ants, the degree of pvERR expression is weaker in mushroom body, accessory lobe and suboesophageal ganglion of male ants, but stronger expression was found in optic lobes.

KeyWords:

Polyrhachis vicina; pvERR; in situ hybridization; insect brain