自然科学版
陕西师范大学学报(自然科学版)
体育科学
长期停训大鼠海马神经元细胞凋亡因子Bax、Bcl-2的表达
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赵永寿1,田振军2*,白建超1
(1 陕西教育学院 体育系, 陕西 西安 710061; 2 陕西师范大学 体育学院, 陕西 西安 710062)
赵永寿,男,副教授,主要从事运动人体科学及体育教育学研究.E-mail:zys02912@126.com.* 通信作者:田振军,男,教授.E-mail:zhenjunt@sunu.edu.cn.
摘要:
以不同强度训练大鼠停训28周为对象,应用免疫组织化学技术,探讨运动对大鼠海马神经元细胞凋亡因子Bax、Bcl-2表达的影响.参照Bedford训练方案,建立起Sprague dawley(SD)大鼠有氧训练与疲劳训练动物模型,停训28周后,观察大鼠海马神经元Bax、Bcl-2的表达变化.结果表明:长期有氧训练大鼠老年期海马神经元Bcl-2/Bax比值升高,长期疲劳训练大鼠老年期海马神经元Bcl-2/Bax比值下降.这是因为长期有氧训练促进了大鼠海马神经元产生了良好的适应,对大鼠老年期海马神经元起到保护和延缓其衰老的作用.而长期疲劳训练会影响到大鼠老年期海马神经细胞的功能状态.
关键词:
运动训练; 海马; 细胞凋亡因子; 停训; 免疫组织化学
收稿日期:
2011-09-15
中图分类号:
G804.5文献标志码: A
文献标识码:
A
文章编号:
1672-4291(2012)01-0104-05
基金项目:
陕西省自然科学基金资助项目(2004C232); 陕西省教育厅科研计划资助项目(08JK018).
Doi:
Expression change of Bax、Bcl-2 in the hippocampus neuron of rats after long-term stop trained
ZHAO Yong-shou1, TIAN Zhen-jun2*, BAI Jian-chao1
(1 Department of Physical Education, Shaanxi Institute of Education, Xi′an 710061,Shaanxi, China;2 College of Physical Education, Shaanxi Normal University, Xi′an 710062, Shaanxi, China)
Abstract:
The influence on the expression of Bax、Bcl-2 in the hippocampus neuron of stopping training 28 weeks rats is studied. Refered to the training scheme of Bedford, the training model of SD rats is established. All rats in the normal cage live 28 weeks and the expression change of Bax and Bcl-2 in the Hippocampus Neuron is observed. The results show that aerobic training promotes the ratio of Bcl-2/Bax in CA1 area and CA3 area of hippocampus, fatigue training restrain the ratio of Bcl-2/Bax in CA1 area and CA3 area of hippocampus after stopping training. It is analyzed that aerobic training has taken good care of the hippocampus neuron of the aged rats, fatigue training has effected the function of the hippocampus neuron of the aged rats.
KeyWords:
sports training; hippocampus; Bax; Bcl-2; stopping training; Immunohisyochemistry