自然科学版
陕西师范大学学报(自然科学版)
生命科学
紫阳富硒茶中茶多糖、碱溶性茶蛋白结构形貌及硒赋存形态
PDF下载 ()
焦自明1,杨建雄2*,张小飞1,窦佩娟1,刘小乔2,郭琦1
(1 陕西师范大学 物理学与信息技术学院;2 陕西师范大学 生命科学学院, 陕西 西安 710062)
焦自明,男,硕士研究生,主要研究方向为天然产物的分离鉴定及功能评价. E-mail:jiao925@163.com.* 通信作者: 杨建雄,男,教授. E-mail:jxyang @snnu. edu. cn.
摘要:
研究了紫阳富硒茶硒赋存形态,用原子力显微镜观测紫阳富硒茶中茶多糖、碱溶性茶蛋白的结构形貌.结果表明:茶多酚硒占总硒的0.89%,蛋白硒占总硒的60.12%,多糖硒占总硒的18.06%,蛋白质组分中又以碱溶性蛋白结合硒含量最高,达到23.81%;观测表明,茶多糖为多侧支的树枝状结构,碱溶性蛋白主要以大小比较均一的杆状或球状结构存在.
关键词:
紫阳富硒茶; 硒; 赋存形态; 原子力显微镜
收稿日期:
2011-05-19
中图分类号:
R151.2文献标志码: A
文献标识码:
A
文章编号:
1672-4291(2012)01-0072-05
基金项目:
国家自然科学基金资助项目(20575039).
Doi:
Structure morphology of tea polysaccharide、alkali-soluble proteinand distribution of Selenium in tea from Ziyang
JIAO Zi-ming1, YANG Jian-xiong2*, ZHANG Xiao-fei1, DOU Pei-juan1, LIU Xiao-qiao2, GUO Qi1
(1 College of Physics and Information Technology, Shaanxi Normal University,2 College of Life Sciences, Shaanxi Normal University, Xi′an 710062, Shaanxi, China)
Abstract:
This study is to investigate the combined forms of selenium in tea from Ziyang county.The structure of tea polysaccharide and alkali-soluble protein are investigated by atomic force microscopy (AFM).The results showed that the ratio of selenium in tea polyphenol is 0.89% in total selenium comtent; the ratio of the selenium in tea protein and in tea polysaccharide is 60.12% and 18.06%,respectively. At the same time, Alkali-soluble protein seems to contain the highest amount of bound selenium, contributing to 23.81% of total selenium content. It is found that tea polysaccharide on mica shaped Multi-lateral branch-like structure by AFM, while the alkali-soluble protein existed in the form of size comparison uniform lever or spherical structure.
KeyWords:
enriched tea of ziyang; selenium; organically bound selenium; atomic force microscopy