绞股蓝多糖脱蛋白工艺及相对分子质量的测定-陕西师范大学学报期刊社网站

陕西师范大学学报（自然科学版）

生命科学

绞股蓝多糖脱蛋白工艺及相对分子质量的测定

周宝珍, 肖娅萍*

（教育部药用植物资源及天然药物化学重点实验室, 陕西师范大学生命科学学院, 陕西西安 710062）

周宝珍，男，硕士研究生，主要研究方向为中药化学.E-mail：zhoubaozhen123@163.com.* 通信作者：肖娅萍，女，教授.E-mail: yapingxiao@snnu.edu.cn.

摘要:

采用Sevag法、木瓜蛋白酶-Sevag法、TCA-Sevag法对绞股蓝粗多糖脱蛋白工艺进行研究.通过DEAE—52纤维素柱层析和葡聚糖凝胶G—200柱层析对粗多糖进行分离纯化，采用高效液相色谱分析法对精制多糖GPM—21的相对分子质量进行测定分析.结果表明：木瓜蛋白酶-Sevag法效果最佳，蛋白脱除率高且多糖损失量小，累计蛋白脱除率可达63.08%，多糖保留率为77.34%.液相渗透色谱法测定GPM—21的相对分子质量为200 576.

关键词：

绞股蓝；多糖；脱蛋白；相对分子质量；高效液相色谱

收稿日期：

2011-05-19

中图分类号：

Q946.3文献标志码： A

文献标识码：

文章编号：

1672-4291(2012)01-0063-04

基金项目：

国家“十一五”科技支撑计划项目（2006BAI06A12—04）.

Doi:

Deproteinization in preparation of Gynostemma pentaphyllum polysaccharide and determination of polysaccharide molecular weight

ZHOU Bao-zhen， XIAO Ya-ping*

（Key Laboratory of Ministry of Education for Medicinal Plant Resource and Natural Pharmaceutical Chemistry，College Life Science，Shaanxi Normal University, Xi′an 710062, Shaanxi, China)

Abstract:

The optimum technologies of deproteinization using Sevag method, Papain-Sevag method and TCA-Sevag method were studied. In addition, crude polysaccharides were isolated and purified by DEAE—52 cellulose column chromatography, and Sephadex G—200 column chromatography, and relative molecular mass of refined polysaccharides GPM—21 were measured by HPLC.The results indicated that papain-Sevag method was the best method for the deproteinization in among of methods.The removal rate of total protein could reach up to 63.08%, and the retention rate of polysaccharide was 77.34%.The relative molecular mass of GPM—21 was 200 576.

KeyWords:

Gynostemma pentaphyllum; polysaccharide; deproteinization; the relative molecular mass; HPLC(High Performance Liquid Chromatography)