Adenoviral DNA vector genetically modified with pⅨ-mCherry was successfully constructed by means of homologous recombination in E.coli. Bj5183. The virus lysates were obtained after the transfection of adenovirus plasmid linearized by Pac Ⅰ into HEK293 cells for 7～10 days. Adenoviral lysates were propagated, and then adenoviral particles labeled with pⅨ-mCherry were purified by CsCl density gradient centrifugation. Purified virus particles were examined under fluorescence microscope, and the localization of fluorescently labeled adenovirus was then investigated by applying purified viruses into A549 cells at different time points. The results indicated that adenovirus particles were found to show red fluorescence under fluorescence microscope. With the prolonged incubation time of fluorescently labeled adenovirus with A549 cells, the viral particles were gradually localized around nucleus.

gene therapy; adenovirus; virus tracking; labeling of adenovirus; capsid protein pⅨ