一氧化氮参与胞外ATP抑制泡桐花粉萌发和花粉管伸长-陕西师范大学学报期刊社网站

陕西师范大学学报（自然科学版）

生命科学

一氧化氮参与胞外ATP抑制泡桐花粉萌发和花粉管伸长

张媛华1，2，张韶杰1，佘小平1*

(1 陕西师范大学生命科学学院，陕西西安710062； 2 渭南师范学院环境与生命科学系，陕西渭南 714000)

张媛华，女，博士研究生，研究方向为植物细胞信号转导.

摘要:

研究了胞外ATP（eATP）对泡桐（Paulownia tomaentosa Steud.）花粉萌发和花粉管伸长的效应及其与一氧化氮（NO）的关系.结果表明，eATP和NO供体硝普钠（SNP）均显著抑制花粉萌发和花粉管伸长，且表现明显浓度和时间依赖效应.一氧化氮合酶（NOS）抑制剂（LNAME）和NO清除剂（cPTIO）能够部分阻止eATP对花粉萌发和花粉管伸长的抑制作用，显示eATP抑制花粉萌发和花粉管伸长与其诱导NO产生有关，eATP诱导的NO可能由NOS催化产生.

关键词：

胞外ATP；泡桐；花粉萌发；花粉管伸长；一氧化氮

收稿日期：

2010-10-15

中图分类号：

Q945.6

文献标识码：

文章编号：

16724291(2011)05006205

基金项目：

陕西省自然科学研究计划项目(2003C102)；渭南师范学院科研基金项目（07YKZ032）.

Doi:

The involvement of nitric oxide in eATPinhibited pollen germination and tube growth of Paulownia tomentosa

ZHANG Yuanhua1，2, ZHANG Shaojie1, SHE Xiaoping1*

(1 College of Life Science, Shaanxi Normal University, Xi′an 710062, Shaanxi, China; 2 Department of Environment and Life Sciences, Weinan Teachers University, Weinan 714000, Shaanxi, China)

Abstract:

The role of eATP and its relationship with nitric oxide (NO) in pollen germination and tube growth of Paulownia tomentosa were studied. The results showed that both eATP and NO donors (sodium nitroprusside) inhibited pollen germination and tube growth in a dosedependence manner. Both NOS inhibitor (LNAME) and NO scavenger (cPTIO) partly reversed eATPinhibited pollen germination and tube growth, indicating that the inhibitory effects of eATP on pollen germination and tube growth are related to NO generated by a NOSlike enzyme in pollen.

KeyWords:

extracellular ATP (eATP); Paulownia tomentosa; pollen germination; pollen tube growth; nitric oxide