Abstract:
In order to investigate the effects of bisphenol A (BPA) in water environment on aromatase（P450arom）and estrogen receptor（ER）in the testis of amphibian，adult male frogs Rana chensinensis were exposed to 5×10-7，5×10-8 mol/L BPA for 30 d during spermatogonium proliferation, spermatocyte meiosis and spermatoleosis stages of the testis，respectively. In addition, 5×10-9，5×10-10 mol/L 17 β-esradiol (E2) were chosen as positive control. The expressios of P450 arom and ER in testis were detected by immunohistochemistry. The results indicated that P450 arom and ER in Leydig and Sertoli cells of the testis were localized with varying degrees of expression. BPA up-regulates the expression of P450 arom and ER in testis of R. chensinensis during the three stages. There was no significant difference in the inductive effects of bisphenol A on P450 arom, while ER was significant different during the three stages, and the strongest effects were showed at the spermatoleosis stage. These results suggest that the effects of bisphenol A on testicular function were carried out by interfering with P450 arom in sex hormone synthesis pathway, and up-regulating the expression of ER.
