自然科学版
陕西师范大学学报(自然科学版)
生命科学
拟南芥肉桂酰辅酶A还原酶(AtCCR1/2)基因的生物信息学分析
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刘晓晶,崔浪军,夏飞,杨章民*
(陕西师范大学 生命科学学院; 陕西师范大学 药用资源与天然药物化学教育部重点实验室;西北濒危药材资源开发国家工程实验室,陕西 西安 710062)
刘晓晶,女,硕士研究生.主要研究方向为基因的表达与调控.E-mail: kikumaru_lxj@163.com.* 通信作者:杨章民,男,副教授,博士.E-mail: yzhangmin@snnu.edu.cn
摘要:
用生物信息学方法分别对拟南芥等植物中两种肉桂酰辅酶A还原酶(CCR)基因序列相似性、编码产物理化性质、三级结构、亚细胞定位、启动子序列、基因表达量以及功能进行预测和分析.结果表明,CCR1和CCR2基因序列、编码产物理化性质和三级结构相似性均较高,编码产物可能定位于细胞质.AtCCR1在拟南芥整株植物和各个器官中的的表达量均远比AtCCR2的高.不同因素处理下AtCCR1和AtCCR2表达状况差异较大,这可能与二者启动子区域含有的顺式作用元件不同有关.
关键词:
拟南芥;肉桂酰辅酶A还原酶;基因表达;生物信息学
收稿日期:
2010-05-12
中图分类号:
Q554.9
文献标识码:
A
文章编号:
1672-4291(2011)03-0067-06
基金项目:
国家自然科学基金资助项目(30870303); 陕西省科技攻关项目(2008K16-02); 国家博士后基金项目(2008043125); 教育部高等学校博士学科点专项科研基金项目(200807181002).
Doi:
Bioinformatic analysis of Cinnamoyl CoA reductase (AtCCR1/2) gene from Arabidopsis thaliana
LIU Xiao-jing, CUI Lang-jun, XIA Fei, YANG Zhang-min*
(College of Life Science; Shaanxi Normal University; Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry; National Engineering Laboratory for Resource Development of Endangered CrudeDrugs in Northwest of China; Xi′an 710062, Shaanxi, China)
Abstract:
Some characteristics of the cinnamoyl CoA reductase (CCR) genes were analyzed and predicted by the tools of bioinformatics. The characteristics were as follows: nucleic acid sequence similarity, promotor sequence, gene expression and function, as well as physical and chemical properties, tertiary structure and subcellular localization of CCR protein. The results showed that AtCCR1 and AtCCR2 sequence, physical and chemical properties, tertiary structure of the two CCR protein were highly similar to that of other plant CCR. The two CCR proteins located to cytoplasm. AtCCR1 gene expression amounts in whole plant and each organs were all higher than that of AtCCR2. Moreover, AtCCR1 and AtCCR2 were differentially expressed in response to several conditions, sunch as salinity, ABA, cycloheximide, et al. The differences were probably correlated with diffrent cis-acting elments in their promoter sequences.
KeyWords:
Arabidopsis thaliana; Cinnamoyl CoA reductase; gene expression; bioinformatics