Cloning and bioinformatics analysis of Cu/Zn-SOD gene from Salvia miltiorrhiza Bunge
JI Yan-yun， HUA Wen-ping， WANG Zhe-zhi*
(Key Laboratory of Ministry of Education for Medicinal Plant Resource and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, Shaanxi Normal University, Xi′an 710062, Shaanxi, China)
Abstract:
Based on the constructed cDNA library, a Cu/Zn-superoxide dismutase gene (Cu/Zn-SOD) from Salvia miltiorrhiza Bunge was cloned by TD PCR. The characteristics including the composition of amino acid sequences, hydrophobicity or hydrophilicity, secondary structure of protein and function of the Cu/Zn-SOD were analyzed and predicted by the tools of bioinformatics.The results showed that SmCu/Zn-SOD is a hydrophilic, non-transmembrane and non-secretory protein. The main motif of Cu/Zn-SOD in secondary structure was random coils and extended strands.Despite sequence variations, the core structure, sequence and activity sites of SmCu/Zn-SOD were highly similar to that of other green plants.
KeyWords:
Cu/Zn-SOD; TD PCR; Salvia miltiorrhiza Bunge; bioinformatics
