太白泡沙参中薯蓣皂苷元含量的测定-陕西师范大学学报期刊社网站

陕西师范大学学报（自然科学版）

化学与材料科学

太白泡沙参中薯蓣皂苷元含量的测定

陈战国，王英杰，李鹏程，闻化，周继梅，李文丽

(陕西师范大学化学与材料科学学院，陕西省大分子科学重点实验室，陕西西安 710062)

陈战国，男，副教授，主要从事有机合成及药物分析方面的研究.E-mail：chzhg@snnu.edu.cn.

摘要:

以高氯酸-香荚兰醛为显色剂, 以冰醋酸为溶剂,建立了光度法定量测定太白泡沙参中薯蓣皂苷元含量的新方法.结果表明，回归方程为Y=3.011 7X-0.003 4，相关系数 r=0.999 8，线性范围 0.050～0.298 mg/mL.利用该方法对太白泡沙参中薯蓣皂苷元含量进行了定量测定，薯蓣皂苷元含量高达8%左右，7次回收率平均值为100.2%，RSD=1.03%.

关键词：

薯蓣皂苷元; 太白泡沙参; 含量测定

收稿日期：

2011-01-18

中图分类号：

O6573

文献标识码：

文章编号：

1672-4291(2011)03-0045-03

基金项目：

陕西省自然科学基金资助项目（2009JM2011）；陕西师范大学研究生创新基金资助项目（2008CXB009）.

Doi:

The determination of diosgenin in the root of the A . potaninii Korsh from Taibai Mountain in China

CHEN Zhan-guo, WANG Ying-jie, LI Peng-cheng, WEN Hua, ZHOU Ji-mei, LI Wen-li

(College of Chemistry and Materials Science, Shaanxi Normal University; Key Labotatory for Macromolecular Science of Shaanxi Province, Shaanxi Normal University, Xi′an 710062, Shaanxi, China)

Abstract:

Photometric method for the determination of diosgenin in the root of the A. potaninii Korsh from Taibai Mountain in China has been developed using perchloric acid-vanillin as colour-developing agent and glacial acetic acid as solvent. The experiment results indicated that the regression equation of the method is Y=3.011 7X-0.003 4 (r=0.999 8), and the linear range is found to be 0.050～0.298 mg/mL. The content of diosgenin in the root of the A. potaninii Korsh from Taibai Mountain in China has been accurately determined by using this method. The content of diosgenin is up to 8 % . The even recovery (n=7) is 100.2% and the relative standard deviation is less than 1.03%.

KeyWords:

diosgenin; A. potaninii Korsh from Taibai Mountain in China; determination of content