Abstract:
The analyse of polysaccharides AP1 and CAP1 extracted from Shaggy Mane by boiling water and ultrasonic process is carried out.The affect of ultrasonic-assisted extraction on the structure of Shaggy Mane polysaccharide AP1 is investigated. The results showed that the components of Polysaccharides AP1 is identified by gas chromatography (GC) as: mannose,glucose,galactose;and CAP1 is xylose,mannose,glucose,galactose;the infrared spectra showed that polysaccharides AP1 and CAP1 have saccharide characteristic absorption peaks in the range from 4 000 to 650 cm-1.The infrared spectrum of AP1 has absorption peaks at 865 cm-1and 764 cm-1,but CAP1 has the peaks at 903 cm-1、 826 cm-1 and 756 cm-1.Atomic force microscopy (AFM) diagram showed the polysaccharides has branch structure.The ultrasound was likely to cause the connection pattern change of monosaccharides,and easily resulting in the formation of relatively uniform mesh structure.