血卟啉在不同培养肿瘤细胞中定量及定位的研究-陕西师范大学学报期刊社网站

陕西师范大学学报（自然科学版）

生命科学

血卟啉在不同培养肿瘤细胞中定量及定位的研究

苏晓敏，王元，朱波，王筱冰，王攀，刘全宏*

(陕西师范大学生命科学学院，陕西西安 710062)

苏晓敏，女，硕士研究生，主要研究方向为医学超声.* 通信作者：刘全宏，男，教授，博士研究生导师.E-mail: lshaof@snnu.edu.cn.

摘要:

在不同温度下，用浓度分别为10 μmol/L、20 μmol/L、40 μmol/L的血卟啉孵育L1210和S180肿瘤细胞，通过荧光分光光度法检测血卟啉在L1210及S180肿瘤细胞内的富集以及温度对其的影响，激光共聚焦扫描显微镜观察血卟啉的亚细胞定位.结果表明，随着孵育浓度的增加和孵育时间的延长，肿瘤细胞对血卟啉的吸收也相应增加，60~120 min细胞内血卟啉含量最高；不同温度也会影响细胞对血卟啉的吸收，37℃为最适孵育温度.

关键词：

L1210细胞； S180细胞；血卟啉；荧光分光光度法；亚细胞定位

收稿日期：

2010-06-01

中图分类号：

Q279

文献标识码：

文章编号：

1672-4291(2011)01-0071-04

基金项目：

国家自然科学基金资助项目（30270383）.

Doi:

Hematoporphyrin retained and subcellullarly located in different tumor cells

SU Xiao-min， WANG Yuan， ZHU Bo， WANG Xiao-bing， WANG Pan， LIU Quan-hong*

(College of Life Science, Shaanxi Normal University, Xi′an 710062, Shaanxi, China)

Abstract:

It have been investigated that hematoporphyrins were retained in L1210 and S180, respeceively. The cells were incubated with hematoporphyrin (HpD) at 10μmol/L，20μmol/L，40μmol/L concentrations and different temperatures. The intracellular HpD concentration and the effect imposed by temperature were measured by the fluorescence photometer, and laser scanning confocal microscope was used to study the subcellular location pattern of HpD. The results showed that the intracellular concentration of HpD increased with the increasing of HpD and incubated time，the intracellular concentration was highest in 60~120 min; the absorption of HpD could also be influenced by temperature，and 37℃ is the most suitable incubation temperature.

KeyWords:

L1210 cell; S180 cell; hematoporphyrin; fluorenscence photometer; subcellular location