Abstract:
One sequence which showed homology with COR413(cold-regulated gene)gene family was found from the expressed sequence tags（EST） of Salvia miltiorrhiza using the BLAST approach. The full-length cDNA and DNA sequence of the gene was cloned by PCR and named as SmCOR413. The DNA sequence was 1708bp, and contained four extrons and three introns. It encoded a predicated polypeptide of 171 amino acids with a molecular weight of 19.77 kDa and a predicated pI of 8.95. Amino acids similarity of SmCOR413 compared with COR413 in other nine plants reached 66%—78%. This gene maybe belonged to COR413 gene family without signal peptide. Real-time PCR result showed that the SmCOR413 expressed in root, stem and leaf. The mRNA of SmCOR413 was the most abundant in leaf and stem, the least in root.
