自然科学版
陕西师范大学学报(自然科学版)
生命科学
壬基酚对中国林蛙生长发育的转代效应
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李辉,李忻怡,张育辉*
(陕西师范大学 生命科学学院, 陕西 西安 710062)
李辉,女,硕士研究生,主要研究方向为动物生殖与发育.* 通信作者:张育辉, 男, 教授, 博士研究生导师. E-mail: yu-huizhang @163.com.
摘要:
为了探讨成体中国林蛙(Rana chensinensis) 暴露于壬基酚(nonylphenol, NP)后对其子代幼体生长发育和性腺分化的转代效应,将产卵前雌性成体分别暴露在浓度为25×10-7、125×10-7、5×10-8 mol/L NP的水体中60 d,将产卵孵化后的26期蝌蚪做两种处理,第一种是在不含NP的水体中分组饲养,第二种是继续暴露在与母体暴露NP浓度相同的水体中,两种处理均至蝌蚪完全变态.结果显示:母体NP暴露处理后对子代蝌蚪的发育时间、体长和体重、雌雄比例的影响均无显著性差异,但对子代蝌蚪继续NP暴露处理的蝌蚪则出现了发育滞后、体长和体重减少、雌性比例升高的现象.说明在浓度为5×10-8~25×10-7 mol/L NP范围的水体中,NP没有干扰成体雌性林蛙的卵子发育,对其子代的生长发育和性腺分化未形成影响.但这个浓度范围的NP可直接影响蝌蚪的生长发育和性腺分化.
关键词:
壬基酚; 中国林蛙; 生长发育; 转代效应
收稿日期:
2009-12-01
中图分类号:
Q548 .2;X174
文献标识码:
A
文章编号:
1672-4291(2010)04-0079-05
基金项目:
国家自然科学基金资助项目 (30770243),陕西省自然科学基础研究计划项目(SJ08C101)
Doi:
Transgenerational effects of nonylphenol on growth and development of frog, Rana chensinensis
LI Hui, LI Xin-yi, ZHANG Yu-hui*
(College of Life Science, Shaanxi Normal University , Xi′an 710062, Shaanxi, China)
Abstract:
In order to understand transgenerational effect of nonylphenol on growth, development and gonadal differentiation of Chinese forest frog, Rana chensinensis, adult females were exposed to nonylphenol (NP) at different concentrations (25 × 10-7, 125 × 10-7, 5 × 10-8 mol/L) for 60 days. The offspring tadpoles were divided into two groups, one group was feed in water without NP, another was treated with NP at stage 26 until completion of metamorphosis. The results indicated that offspring tadpoles in the group that was not treated with NP showed no significant change in the development time, body length, body weight and sex ratio as compared with control group. However, the offspring tadpoles in the group with NP treatment, showed significant decrease in developmental velocity, body length and body weight, and significant increase in percentage of females. It could be concluded that there was no transgenerational effect of nonylphenol(5×10-8~25×10-7 mol/L NP)on growth and development of Chinese forest frog, and that NP could directly affect the growth, development and gonadal differentiation of tadpoles.
KeyWords:
nonylphenol; Rana chensinensis; growth and development; transgenerational effects