两组浓度的蛋白酶体抑制剂MG132诱导人宫颈癌细胞Hela凋亡-陕西师范大学学报期刊社网站

陕西师范大学学报（自然科学版）

生命科学

两组浓度的蛋白酶体抑制剂MG132诱导人宫颈癌细胞Hela凋亡

贾红玲，葛峰，李丽萍，卢春花

(暨南大学生命与健康工程研究院，广东广州 510632)

贾红玲，女，实验师，主要研究方向为分子生物学和蛋白组学．E-mail: jiahongling@yahoo.com.cn.

摘要:

为了探讨不同浓度蛋白酶体抑制剂MG132在诱导人宫颈癌Hela细胞凋亡过程中作用，分别以两组浓度的蛋白酶体抑制剂MG132处理人宫颈癌细胞Hela，通过MTT法检测Hela细胞活力，应用Annexin Ⅴ和PI 双染的细胞流式法检测Hela细胞凋亡率，应用Western blotting和酶标仪法检测Hela细胞内Caspase-3活性变化的情况．结果表明：低浓度MG132诱导Hela细胞凋亡不明显，高浓度MG132诱导Hela细胞凋亡明显；不同浓度蛋白酶体抑制剂MG132诱导人宫颈癌Hela细胞凋亡的程度存在明显差异．

关键词：

蛋白酶体抑制剂MG132；人宫颈癌Hela细胞；细胞凋亡

收稿日期：

2009-10-15

中图分类号：

Q291

文献标识码：

文章编号：

1672-4291(2010)03-0065-05

基金项目：

国家自然科学基金资助项目（30400071）.

Doi:

Apoptosis in Human cervix cancer Hela cells induced by two group concentrations of proteasome inhibitor MG132

JIA Hong-ling, GE Feng, LI Li-ping, LU Chun-hua

(Institutes of Life and Health Engineering，Jinan University，Guangzhou 510632，Guangdong,China)

Abstract:

To investigate the effect of different concentration proteasome inhibitor (MG132) on the apoptosis of human cervix cancer Hela cells, Hela cells was cultured with different concentrations of proteasome inhibitor (MG132), and the cell viability was determined by MTT assay. The cell apoptosis rate was determined by Annexin Ⅴ/PI staining and flow cytometry. Caspase-3 activity change was tested by Western blotting and ELISA Reader. The results indicate that high concentration of proteasome inhibitor (MG132) is more significant in injury and apoptosis of Hela cells than low concentration one. The different concentration of proteasome inhibitor (MG132) is obvious in inducing apoptosis of hela cells.

KeyWords:

proteasome inhibitor MG132; Human cervix cancer Hela cells; cell apoptosis