HPLC分析秦艽多糖的单糖组成-陕西师范大学学报期刊社网站

陕西师范大学学报（自然科学版）

生命科学

HPLC分析秦艽多糖的单糖组成

陈克克，王吉吉之*

(陕西师范大学教育部药用植物资源与天然药物化学重点实验室，陕西西安 710062)

陈克克，女，助教，硕士，主要从事中药活性成分分析的研究．*通讯作者：王吉吉之，男，教授，博士研究生导师．E-mail：zzwang@snnu.edu.cn.

摘要:

采用水提醇沉法提取陕西产秦艽多糖，经三氟乙酸水解，水解产物用衍生化试剂1-苯基-3-甲基-5-吡唑啉酮柱前衍生化后进行高效液相色谱分析．结果表明，秦艽多糖含有甘露糖、鼠李糖、葡萄糖醛酸、半乳糖醛酸、葡萄糖、半乳糖和阿拉伯糖七种单糖，其摩尔比为0.06∶0.25∶0.03∶1.00∶1.23∶0.87∶3.52．

关键词：

秦艽多糖；单糖组成；衍生化；高效液相色谱

收稿日期：

2009-01-15

中图分类号：

Q946.3

文献标识码：

文章编号：

1672-4291(2010)01-0079-03

基金项目：

国家科技支撑计划项目（2006BAI06A15）

Doi:

Component analysis of monosaccharides composition in polysaccharide from the root of Gentiana macrophylla Pall. by HPLC

CHEN Ke-ke, WANG Zhe-zhi*

(Key Laboratory of Ministry of Education for Medicinal Plant Resource and Natural Pharmaceutical Chemistry, Shaanxi Normal University, Xi′an 710062, Shaanxi, China)

Abstract:

The polysaccharide from the root of Gentiana macrophylla Pall. was extracted by hot distilled water, and then precipitated by alcohol, and hydrolyzed by TFA. The hydrolysate derivatizated with PMP and the PMP derivatives were analysed by HPLC. Results showed that the polysaccharide was consisted of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose and arabonose, their molar ratio was 0.06∶0.25∶0.03∶1.00∶1.23∶0.87∶3.52.

KeyWords:

polysaccharide from the root of Gentiana macrophylla Pall.; monosaccharide composition; derivatization; high performance liquid chromatography