自然科学版
陕西师范大学学报(自然科学版)
生命科学
连翘种子油GC-MS分析及抗氧化活性研究
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魏希颖,徐慧娴,杨小军,陈康健,张玲,葛茜,曾成鸣
(陕西师范大学 生命科学学院, 陕西 西安 710062)
魏希颖,女,副教授,主要从事天然产物的开发与利用方面的研究.E-mail:xiyingwei@snnu.edu.cn.
摘要:
利用GC-MS联用技术对连翘种子油成分进行分析,各成分的质谱图与标准谱对照,分离鉴定出63种化合物,其中萜类化合物占70%以上.所鉴定成分的含量占全油的98.91%.主要成分为β-蒎烯(47.78%),其次是β-水芹烯(14.91%)和α-蒎烯(14.02%),另检测出0.76%的含碘化合物和0.55%的含硅化合物等.用DPPH法和邻苯三酚自氧化法测定其抗氧化活性,结果发现其对有机自由基(DPPH)和超氧阴离子自由基(O·2)均有显著清除作用,清除率高达90%以上.
关键词:
连翘种子油; GC-MS分析; 抗氧化活性
收稿日期:
2009-10-15
中图分类号:
R282.7
文献标识码:
A
文章编号:
1672-4291(2010)01-0070-05
基金项目:
国家科技支撑计划子课题项目(2006BA106A-04)
Doi:
Analysis of essential oil of Forsythia suspense (Thunb.) Vahl. by GC-MS and its antioxidant activities
WEI Xi-ying, XU Hui-xian, YANG Xiao-jun, CHEN Kang-jian, ZHANG Ling, GE Qian, ZENG Cheng-ming
(College of Life Sciences, Shaanxi Normal University, Xi′an 710062, Shaanxi, China)
Abstract:
The essential oil from seeds of Forsythia suspense (Thunb.) Vahl. was analyzed by GC-MS, and 63 components were identified by comparision with the standard mass spectrogram. Among of the components, the terpenoid was more then 70 percent. The content of indentified componenes was 98.91 percent of the whole oil. The main chemical components of the oil were β-Pinene(47.78%),β-Phellandrene(14.91%) and α-Pinene(14.02%).The iodine and silicon components were also identified in 0.76% and 0.55%, respectively. The antioxidant activities of the oil were assayed through scavenging effects to DPPH and superoxide anion free radical (O·2). The results indicated that essential oil from seeds of Forsythia suspense (Thunb.) Vahl. showed much high antioxidant activity and the scavenging effects to DPPH and (O·2) were more than 90%. The study could provide theoretical foundation for further studying and developing of the essential oil from seeds of Forsythia suspense (Thunb.) Vahl.
KeyWords:
essential oil from seeds of Forsythia suspense (Thunb.) Vahl.;GC-MS; antioxidantive activitie