Abstract:
To investigate the effects of ANXA2 knockdown on cell proliferation, apoptosis and migration of Caco-2 cells, four siRNAs targeting ANXA2 were designed and cloned, then the recombinant plasmids were transfected into Caco-2 cells, the changes of ANXA2 expression levels were detected by RT-PCR and immunocytochemistry respectively. The results showed that the four siRNAs, especially site 1 siRNA, could inhibit the expression of ANXA2 effectively. Over 70% ANXA2 expression was inhibited at 72 h post transfection. The results indicate that the proliferation, attachment and motility of Caco-2 cells can be significantly inhibited when the gene expression was knockdown. It is concluded that ANXA2 is closely associated with the development of colorectal carcinoma, and it plays important roles in regulating the Caco-2 cell proliferation and motility, a probable mechanism is that ANXA2 enhances the DNA synthesis and regulates the actin function of Caco-2 Cells.
