Abstract:
The total polysaccharides in the root of the A.potaninii Korsh from Taibai Mountain in China was determinated. The stock solution of the total polysaccharides was prepared from the extraction with water, and then the extraction solution was treated by alcohol to eliminate protein. Based on the optimization of conditions including testing wave length, amoune of reagents and reaction temperature et al, the method of phenol-sulfuric acid was established for determination of the total polysaccharide in the A.potaninii Korsh. The tests induding accuracy, reproducibility and recovery rate have been carried out. When the concentration of control solution containing glucose was 0.1 mg/mL, the 1.0 mL of 5 %-phenol aqueous solution and 5.0 mL of concentrated sulfuric acid were necessary to complete the reaction at room temperature. The results are as follows. The RSD of accuracy was 0.66 % (n=5), RSD of reproducibility was 0.29 % (n=5), and average recovery rate was 99.9 % (n=9). The results indicated that the method is simple, reliable to determine the total polysaccharide in the A.potaninii Korsh.
