Five different solvents were used to extract oil from silkwormpupa by Soxhlet′s extraction method. In order to increase the yield of protein extracted from silkwormpupa, the technology parameters were optimized by the method of dissolving with alkali and depositing with acid. The experiments of quadratic regression orthogonal rotary design were used to investigate the effects of some factors such as pH value, the ratio of material to solvent and extracting temperature on the yield of protein. The results were as follows: N-hexane was the best extracting solvent, the extraction percentage 28.9%; the optimum extracting conditions were that pH value 10, the ratio of material to solvent 1:12, extracting temperature 60℃ and extracting time 90 min. The yield of protein was 48.22% after double-extracted continuously. Moreover, the isoelectric point of pupa protein was about pH4.4,and the purity coefficient of the pupa protein was 95.2% after frozen-drying.

silkwormpupa； protein； extracting