Effect of fusicoccin-induced stomatal opening on hydrogen peroxide in guard cells of Vicia faba
ZHANG Shao-jie, HAN Xi-zhu, SHE Xiao-ping*
(College of Life Sciences, Shaanxi Normal University, Xi′an 710062, Shaanxi, China)
Abstract:
To investigate the relationship between hydrogen peroxide (H2O2) levels in guard cells and stomatal opening induced by fusicoccin (FC) in Vicia faba. The results indicated that stomatal opening in darkness induced by FC, ascorbic acid (ASA), diphenylene iodonium (DPI), and NADPH oxidase was obvious. Additionally, FC and ASA not only prevented stomatal closure induced by exogenous H2O2 but also promoted the stomatal closed by dark to reopen. Howerer, the similar phenomena were not observed in presence of DPI. Based on the results mentioned above, that FC probably reduce the levels of H2O2 in guard cells by scavenging directly to induce stomatal opening in darkness.
KeyWords:
fusicoccin； stomatal； guard cell； hydrogen peroxide
