自然科学版
陕西师范大学学报(自然科学版)
生命科学
响应面法优化超声-酸水解法提取薯蓣皂素工艺研究
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牛春玲,吴胜举*,沈壮志,郝长春
(陕西师范大学 物理学与信息技术学院, 陕西 西安 710062)
牛春玲,女,硕士研究生,研究方向为生物物理学.
摘要:
采用先超声后水解的方法,从黄姜中提取薯蓣皂素.利用响应面分析法(response surface methodology, RSM) 优化超声提取的工艺条件.实验结果表明,对于所研究的10 g黄姜,最佳提取乙醇体积分数为72%,溶剂体积为100 mL,超声时间为45 min,超声功率为420 W.与发酵-酸水解法提取薯蓣皂素相比,本方法提取率提高了26.46%.
关键词:
响应面分析法;超声;提取;薯蓣皂素
收稿日期:
2008-05-08
中图分类号:
O4262
文献标识码:
A
文章编号:
1672-4291(2009)01-0074-04
基金项目:
国家自然科学基金资助项目(10074042;10474061)
Doi:
Optimization for ultrasonic-acid hydrolysis extraction of diosgenin by response surface methodology
NIU Chun-ling, WU Sheng-ju*, SHEN Zhuang-zhi, HAO Chang-chun
(College of Physics and Information Technology, Shaanxi Normal University, Xi′an 710062, Shaanxi, China)
Abstract:
Diosgenin is extracted by ultrasonic extracting and hydrolyzing method from Dioscorea Zingiberensis Wright. By response surface methodology (RSM), the extraction conditions are optimized. The result shows that the optimum extraction conditions obtained for 10 g Dioscorea Zingiberensis Wright are as follows: ethanol concentration of 72 %, ethanol volume of 100 mL, ultrasonic time of 45 min and ultrasonic power of 420 W. The model obtained by RSM is well matched with practice conditions. Extraction yield of ultrasonic extraction is increased 26.46% compared with that of the traditional method.
KeyWords:
response surface methodology;ultrasonic;extraction;diosgenin