Preparation of gelatin microspheres with different surface structures and the effect of surface morphology on cell proliferation
ZHANG Shu-xia1, WANG Yi-juan1, HUANG Sha2, JIN Yan2, LIU Shou-xin1*, FANG Yu1
(1 Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education;College of Chemistry and Materials Science, Shaanxi Normal University, Xi′an 710062, Shaanxi, China;2 College of Stomatology, Fourth Military Medical University, Xi′an 710032, Shaanxi, China)
Abstract:
Natural biodegradable gelatin materials used as raw material and glutaraldehyde used as cross-linking agent, gelatin microspheres with micron-level were prepared by an improved emulsified cold-condensation method. The average diameter of the microspheres was 250 μm. The gelatin microspheres with the different surface structures were obtained by using three different post-treatments. The gelatin microspheres treated by freeze-drying were porous, and it was found that the fibroblasts grew well on the surface of the porous microspheres, and had an obvious proliferation. It could be concluded that the microspheres treated by freeze-drying are suitable to be sued as cell microcarrier.
KeyWords:
gelatin microspheres;cell microcarrier;fibroblast
