不同盐析剂对羔羊皱胃酶盐析效果的影响-陕西师范大学学报期刊社网站

陕西师范大学学报（自然科学版）

食品科学

不同盐析剂对羔羊皱胃酶盐析效果的影响

吴海1，张富新1，昝林森2，李传扬1

(1 陕西师范大学食品工程与营养科学学院，陕西西安 710062；2 西北农林科技大学动物科学学院，陕西杨凌 712100)

吴海，女，硕士研究生，研究方向为畜产品加工与贮藏．

摘要:

以NaCl、Na2SO4、MgSO4和(NH4)2SO4为盐析剂，对影响羔羊皱胃酶的盐析饱和度、盐析pH、盐析温度和盐析时间进行了研究．结果表明，不同盐析剂对羔羊皱胃酶盐析效果各不相同，(NH4)2SO4是盐析羔羊皱胃酶较适宜的盐析剂．(NH4)2SO4饱和度95%、盐析pH值为4.60、盐析温度为4℃下盐析14～20 h，盐析活性回收率可达到92.86%．

关键词：

盐析剂；羔羊皱胃酶；盐析条件

收稿日期：

2007-10-08

中图分类号：

O4261

文献标识码：

文章编号：

1672-4291(2008)04-0106-03

基金项目：

国家“十一五”科技支撑计划奶业重大专项研究基金资助项目(2006BAD04A11)

Doi:

Influences of different salting-out reagents on effect of kid rennet

WU Hai-yue1, ZHANG Fu-xin1, ZAN Lin-sen2, LI Chuan-yang1

(1 College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi′an 710062, Shaanxi, China; 2 College of Animal Science, Northwest Science Technology University of Agriculture and Frestry, Yangling 712100, Shaanxi, China)

Abstract:

Using NaCl、Na2SO4、MgSO4 and (NH4)2SO4 as salting-out reagents,some factors,such as saturation pH, temperature and time, influencing salting-out effects on kid rennet were studied systematically. The results showed that different salting-out reagents have different effect on salting-out and (NH4)2SO4 is a optimal salting-out reagent,by which enzyme activity recovery can reached 9286% under (NH4)2SO4 saturation 95%、 pH 4.60, temperature 4℃ and time 14～20 hours.

KeyWords:

salting-out reagent; kid rennet; salting-out condition