自然科学版
陕西师范大学学报(自然科学版)
生命科学
脱脂沙苑子乙醇提取物的体外抗氧化活性研究
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王莉1,刘静2,杨建雄1*
(1 陕西师范大学 生命科学学院, 陕西 西安 710062; 2 陕西教育学院 生物学系, 陕西 西安 710061)
王莉,女,硕士研究生,主要从事天然产物分离与功能评价方面的研究.
摘要:
通过测定脱脂沙苑子乙醇提取物(ESA)对1,1-二苯基-2-苦苯肼自由基(DPPH·)、超氧阴离子自由基(O-·2)和羟基自由基(·OH)的清除能力、对Fe2+诱导的脂质过氧化反应的抑制作用、还原力和总抗氧化力,评价ESA的体外抗氧化活性,并与公认的抗氧化剂维生素C作了对照.结果表明,ESA有一定的DPPH·清除能力(IC50=4191 μg/mL)、较强的超氧阴离子清除能力(IC50=1179 μg/mL)和羟基自由基清除能力(IC50=2244 μg/mL),能有效的抑制脂质过氧化(IC50=170 mg/mL),有良好的总抗氧化力,说明沙苑子具有明显的抗氧化活性.
关键词:
沙苑子; 抗氧化; 自由基; 脂质过氧化
收稿日期:
2007-10-08
中图分类号:
R151.2
文献标识码:
A
文章编号:
1672-4291(2008)04-0072-04
基金项目:
国家自然科学基金资助项目 (20575039).
Doi:
Studies on antioxidant activities of ethanoic extract from defatted Semen astragali complanati in vitro
WANG Li1, LIU Jing2, YANG Jian-xiong1*
(1 College of Life Science, Shaanxi Normal University, Xi′an 710062, Shaanxi, China;2 Department of Biology, Shaanxi Education College, Xi′an 710061, Shaanxi, China)
Abstract:
In-vitro antioxiant activities of ethanoic extract from defatted Semen astragali complanati (ESA) have been investigated employing antioxidant activities in different systems including free radical scavenging activities on α,α-diphenyl-β-picrylhydrazyl (DPPH·),super anion radical(O-·2), and hydroxyl radical(·OH), inhibition activity of Fe2+ induced lipid peroxidation, reducing power, and total antioxidant capacity, and the antioxidant activities have been compared with standard antioxidant L-ascorbic acid. The results show that ESA has a certain scavenging activity of DPPH· (IC50=4191 μg/mL), a strong to super anion radical (IC50=1179 μg/mL) and hydroxyl radical(IC50=2244 μg/mL), a good inhibition activity against the lipid peroxidation(IC50=170 mg/mL), and an excellent total antioxidant capacity. As a result, ESA has strong antioxidant activities.
KeyWords:
Semen astragali complanati; antioxidant activity; free radical; lipid peroxidation