Abstract:
The extraction and detection of F1-ATP synthase from pig-liver mitochondria are investegated by using ultrasonic and differential centrifugal methods. The specific activity of F1-ATP synthase in both primary and purified solutions are measured first, then the in-gel enzymatic anylysis are made after polyacrylamide gel electrophoresis. The results show that the specific activity of F1-ATP synthase in purified solution is increased compared with that of the primary solution.
