CHEN Bing1, TANG Liang2*
(1 Scieme and Research Section, Xi′an Institute of Physical Education, Xi′an 710068, Shaanxi, China; 2 Institute of Sports Biology, Shaanxi Normal University, Xi′an 710062, Shaanxi, China)
Abstract:
To find an fast and effectual method for total RNA extracted from micro skeletal muscle tissues of athlete, the traditional Trizol method for total RNA extraction was improved, and the quality of the extracted total RNA was analysed by agarose electrophoresis and optical density test. The results show that total RNA extracted by the improved method has obvious three bands of 28S, 18S and 5S. The special bands expanded by RT-PCR is consistent with β-actin genes. RNA extracted by the method can meet the demands of gene cloning and of biomolecular research in yield and purity of total RNA. The improved method has an important reference to further study the regulating mechanisms about exercise-related genes expression.
KeyWords:
total RNA; extraction; skeletal muscle; β-actin gene; athlete
