The cells were incubated with hematoporphyrin Hp at different concentrations respectively. After then, the intracellular Hp concentration was measured by the fluorescence photometer and estimated based on the standard graph. The results showed that the intracelluar concentration of Hp would increase when Hp concentration and the incubated time were increased. The ability in assimilated Hp by S180 was sligthly stronger than that by Eac. Additionally, uptake of Hp by S180 and Eac were obviously greater than that by H22. There absorption differences would be attributed to the different cell nature.

fluorescence photometer； hematoporphyrin (Hp)； Sarcoma 180 tumor cell； Ehrlich ascites tumor cell； hepatoma H22 cell