LIU Ji-lan1, LU Run-chao2, XIAO Jin-xin2, GAO Ling-xiang1, GAO Zi-wei1
(1 College of Chemistry and Materials Science, Shaanxi Normal University, Xi′an 710062, Shaanxi China；2 College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China)
Abstract:
The phase behaviors of the interactions between six anionic surfactants and lysozyme as functions of head group, hydrocarbon chain length of surfactants and salt concentration have been investigated by UV absorbance at pH=7. Anionic surfactants of surfactants used include CnH2n+1 SO4Na and CnH2n+1 SO3Na(n＝8、10、12). CnH2n+1 SO4 and CnH2n+1SO3 represent sodium alkyl sulfate and sodium alkyl sulfonate, respectively. The result indicates that the interaction of lysozyme with sodium alkyl sulfate is stronger than that with sodium alkyl salfonate at the same hydrocarbon chain length. While the interaction between anionic surfactants and lysozyme became stronger with the increase of the hydrocarbon chain length at the same head group. The interaction became weaker when the salt concentration ranged from 0 mmol/L to 50 mmol/L. When the salt concentration was increased to 100 mmol/L, the interaction was enhanced.
KeyWords:
lysozyme; surfactant; phase behavior; protein
