Abstract:
The role and relationship of NO and H2O2 involved in the stomatal movement of Vicia faba caused by osmotic stress and exogenous abscisic acid (ABA) were investigated by epidermal strip bioassay and laser-scanning confocal microscopy. The results showed that the generation of endogenous NO and H2O2 in guard cells and stomata closure were promoted by osmotic stress and exogenous ABA. Stomata closure and the generation of endogenous NO in guard cells were induced by exogenous H2O2, while exogenous SNP can induce the generation of endogenous H2O2 in guard cells and stomata closure. The relationship of ABA, NO and H2O2 involved in the stomatal movement of Vicia faba caused by osmotic stress was also discussed. The generation of NO and H2O2 under osmotic stress, which indinced the stomata closure, was probably via the presence of ABA, and there was a cross talk between NO and H2O2.
