TaNHX2基因植物表达载体的构建及在烟草中的表达-陕西师范大学学报期刊社网站

陕西师范大学学报（自然科学版）

专题研究

TaNHX2基因植物表达载体的构建及在烟草中的表达

原江锋1，杨建雄1，俞嘉宁2*，王丽娟3，张志琪1，王吉吉之1

(1 陕西师范大学药用植物资源与天然药物化学教育部重点实验室; 2 陕西师范大学生命科学学院, 陕西西安 710062; 3 宁夏大学生物学系，宁夏银川 750021)

原江锋，女，博士研究生，主要从事天然药物化学及功能评价方向的研究．

摘要:

将TaNHX2基因重组于质粒pBIN438的CaMV 35S启动子下游，构建含TaNHX2基因的植物双元表达载体pBIN438-TaNHX2．采用农杆菌介导转化烟草（Nicotiana tobacum L.），获得含TaNHX2的转基因烟草植株．经PCR、RT-PCR分析表明，TaNHX2基因已整合到烟草中，并且得到表达．耐盐分析表明，外源基因TaNHX2提高了转基因植株的耐盐性．

关键词：

TaNHX2；载体构建；烟草；基因表达；抗逆性

收稿日期：

2006-06-15

中图分类号：

R394.33

文献标识码：

文章编号：

1672-4291(2007)03-0078-05

基金项目：

国家自然科学基金资助项目(J2002-B-001)

Doi:

Studies on construction of TaNHX2 gene plant expression vector and Agrobacterium tumefaciens-mediated genetic transformation of Nicotiana tobacum L.

YUAN Jiang-feng1, YANG Jian-xiong1, YU Jia-ning2*, WANG Li-juan3, ZHANG Zhi-qi1, WANG Zhe-zhi1

(1 Key Laboratory of Medicinal Plant Resources and Nature Pharmaceutical Chemistry Ministry of Education, Shaanxi Normal University； 2 College of Life Science, Shaanxi Normal University, Xi′an 710062, Shaanxi China；3 Department of Biology，Ningxia University, Yinchuan 750021, Ningxia, China)

Abstract:

The TaNHX2 gene was constructed in plant bivalent expression vector pBIN438 to obtain a plant expression vector pBIN438-TaNHX2, in which the TaNHX2 gene was constructed at downstream CaMV 35S promoter. Based on the results mentioned above, the vector pBIN438-TaNHX2 was introduced into Nicotiana tobacum L. by Agrobacterium tumefaciens-mediated genetic transformation，and transgenic plantlets were obtained. The successful integration and expression of the gene was identified by PCR and RT-PCR. The results from determination of salinity resistance indicated that the salinity tolerance of transgenic plants was enhanced.

KeyWords:

TaNHX2; construction of vector; Nicotiana tobacum L; gene expression; stress tolerance