Abstract:
TBA detection, colorimetry and fluorescence polarization were used to investigate plasma membrane lipid peroxidation, free fatty acid and membrane fluidity of ascites S180 tumor cells after sonodynamic therapy treatment. Based on the experimental results, the effect of SDT on configuration, function of S180 tumor cells, and the mechanism of SDT for antitumor were discussed. The results indicate that ultrasound alone could initiate lipid peroxidation and lecithoid configuration degradation resulting in membrane fluidity decline. Additionally, in presence of HPD, ultrasonic efficiency could be enhanced obviously. HpD alone had no the effect. After SDT treatment, there is the negative correlation between membrane fluidity declining and lipid peroxidation, as well as lecithoid configuration degradation, and lecithoid configuration degradation may play an important role. According to the results, SDT can elevate the degree of lipid peroxidation, enhance lecithoid configuration degradation to result in the decrease in the membrane fluidity. Consequently, the membrane function is impacted and tumor growth is inhibited availably.
