FAN Juan1, WANG Lin-fang1, KONG Qin2
(1 College of Chemistry and Materials Science, Shaanxi Normal University, Xi′an 710062, Shaanxi, China; 2 College of Materials and Chemistry and Chemical Engineering, Chengdu University of Technology, Chengdu 610059, Sichuan, China)
Abstract:
Absorption, fluorescence spectra and viscometric studies have been carried out on the interactions between genistein and its Sm (Ⅲ) complexes, SmL2·OAc·4H2O ［L= genistein (5-OH group deprotonated)］ with calf thymus DNA. The increase in fluorescence was observed for the Sm (Ⅲ) complex in the presence of DNA, whereas the decrease in fluorescence appeared for genistein. Fluorescence quenching was observed for the DNA-EB system when the Sm (Ⅲ) complex was added, whereas slightly quenched fluorescence was seen for the DNA-EB system with addition of genistein. The relative viscosity of DNA system increased with the addition of the complex. Hypochromism and a small shift were observed in the UV spectra of Sm (Ⅲ) complex in the presence of DNA. The above results suggested that genistein and SmL2·OAc·4H2O could both bind to DNA, but the binding affinity of Sm (Ⅲ) complex much higher than that of genistein. Antitumor activities for the Sm (Ⅲ) complex and genistein were tested by the MTT method. The results show that the inhibitory ratio of the Sm (Ⅲ) complex against the tested tumor cells was higher than that of genistein.
KeyWords:
genistein; Sm (Ⅲ) complex; ctDNA; DNA binding
