豇豆钙调蛋白cDNA的克隆及序列分析-陕西师范大学学报期刊社网站

陕西师范大学学报（自然科学版）

专题研究

豇豆钙调蛋白cDNA的克隆及序列分析

张成，王吉吉之*

(陕西师范大学生命科学学院，陕西西安 710062)

张成，男，硕士研究生,主要从事基因克隆方面的研究工作．

摘要:

从豇豆成熟叶片中提取总RNA，反转录合成cDNA第一链，根据钙调蛋白结构基因两端保守序列设计引物，PCR扩增豇豆钙调蛋白基因，克隆到T-easy载体上并测定了其全序列．序列分析结果表明，豇豆钙调蛋白基因由450个核苷酸组成，编码150个氨基酸．与已知的多种植物钙调蛋白基因相比有很高的相似性，核苷酸序列相似性在80%以上，编码的氨基酸序列相似性在90%以上．

关键词：

豇豆;钙调蛋白;cDNA克隆;序列分析

收稿日期：

2005-12-12

中图分类号：

Q754

文献标识码：

文章编号：

1672-4291(2006)02-0088-04

基金项目：

国家十五科技攻关项目（2004BA721A-35）

Doi:

Isolation of a calmodulin cDNA from Vigna unguiculata (L.) Walp and its sequence analysis

ZHANG Cheng, WANG Zhe-zhi

(College of Life Science, Shaanxi Normal University, Xi′an 710062, Shaanxi, China)

Abstract:

The total RNA was isolated from mature leaves of Vigna unguiculata (L.) Walp. The CaM gene was amplified by PCR after reverse transcription with the conserved primer sequences. A band of 450bp in length of PCR product was subsequenuly cloned with T-easy vector. The result showed that the sequence shared more than 80% and 90% similarity with the calmodulian cDNAs of several other plants in the nucleotide sequence and deduced amino acid sequence, respectively. The isolated CaM clone will enable us to investigate further possible functions of CaM and CaM gene.

KeyWords:

Vigna unguiculata (L.) Walp; calmodulian; cDNA clone；sequence analysis