Chemiluminescence detection of DNA damage induced by formaldehyde and acetaldehyde
WANG Xiao-juan, Qi Hong-lan, ZHANG Cheng-xiao
(Collage of Chemistry and Materials Science, Shaanxi Normal University, Xi′an 710062, Shaanxi, China)
Abstract:
A simple and sensitive chemiluminescence (CL) method for the detection of DNA damage has been developed, Acridinium ester was taken as an intercalator for DNA double-strands and indicator for CL. 0.1 mol/L HNO3 containing 0.3% H2O2 and 0.3 mol/L NaOH containing 2% TritonX—100 were taken as CL stimulated reagent. The behaviors of DNA damage induced by formaldehyde, acetaldehyde were evaluated. It was found that formaldehyde could induce DNA strand breakage in low concentration and made DNA-DNA crosslink in high concentration. Acetaldehyde, however, only caused DNA strand breakage. The behavior and mechanism of DNA damage induced by formaldehyde and acetaldehyde were investigated
KeyWords:
formaldehyde; acetaldehyde; DNA damage; chemiluminescence
