WEI Yue, ZHANG Zhu-jun*, SUN Yong-hua, ZHANG Yan-tu
(Collage of Chemistry and Materials Science, Shaanxi Normal University, Xi′an 710062, Shaanxi, China)
Abstract:
In alkaline solution, the complex of Cobalt (Ⅱ) with icariin can greatly catalyze the chemiluminescent reaction(CL) between Luminol and H2O2. Based on this, a novel HPLC-CL detection method for the determination of icariin was developed. The proposed method has been applied satisfactorily to the determination of icariin in Anshen Bunao mixture. The linear range for the determination of icariin was 0.5～20 μg/mL (r=0.998 6), and the detection limit was 2×10-7g/mL（3σ）. The relative standard deviation was 4.5%(c=2×1010-6 g/mL, n=11).
KeyWords:
high performance liquid chromatography; chemiluminescence; luminol; icariin; Co(Ⅱ)complex
