Genome-wide identification and expression analysis of monodehydroascorbate reductase (MDHAR) family genes in Salvia miltiorrhiza
CHEN Chen1, 2, HAN Limin2, 3, YANG Xiaotong3, WANG Zhezhi2*
(1 Shaanxi Engineering Research Centre for Conservation and Utilization of Botanical Resources,Xi′an Botanical Garden of Shaanxi Province(Institute of Botany of Shaanxi Province), Xi′an 710061, Shaanxi, China; 2 School of Life Sciences, Shaanxi Normal University, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, Xi′an 710119, Shaanxi, China; 3 College of Life Sciences and Food Engineering, Shaanxi Xueqian Normal University,Xi′an 710100, Shaanxi, China)
Abstract:
Ascorbic acid (AsA) is an important factor to remove reactive oxygen when plants response to environment stresses. The antioxidant enzyme mononodehydroascorbate reductase (MDHAR) catalyzes the reduction of ascorbic acid and plays a vital role in maintaining the dynamic balance of ascorbic acid in plant cells. In this study, four MDHAR family genes (SmMDHARS) were identified based on the genome database of Salvia miltiorrhiza. Bioinformatic tools were used to analyze the gene structures, conserved domains, cis-acting elements in promoters of the four genes, to add to the physical and chemical properties, subcellular localization and spatial structures of their encoded proteins. In addition, expression patterns of SmMDHARs in different tissues and different stress environments were determined using qRT-PCR. The results showed that SmMDHARs encode 384~558 amino acids and contain the conserved Pyr_redox-2 domain. SmMDHAR1、2、3 locate in chloroplast while SmMDHAR4 locates in cytoplasm. The cis-acting elements responding to hormones and stresses were found in the promoters of SmMDHARs. The results of SmMDHARs expression patterns showed that SmMDHAR3 was highly expressed in root, and SmMDHARs displayed diverse expression patterns under different stress treatments. Our study preliminarily revealed the structural characteristics and expression patterns of SmMDHARs in Salvia miltiorrhiza and provides an important reference for further elucidating the SmMDHARs function.
KeyWords:
Salvia miltiorrhiza; MDHAR family; bioinformatics；gene expression；resistance
