Abstract:
Tartary buckwheat is a kind of minor cereal crops with rich flavonoids and strong stress-resistance ability. In this study, a NAC transcription factor FtNAC11 was selected and cloned from the transcriptome data of Tartary buckwheat, which is 774 bp long and encodes 275 amino acids. Bioinformatics analysis showed that FtNAC11 belongs to TERN subgroup and has highly conserved domains of 150 amino acids at N-terminal and highly specific C-terminal. It is an unstable hydrophilic protein with no transcriptional activation activity. Predictive analysis of the promoter elements showed that FtNAC11 promotor has a variety of cis-elements, such as light response elements, ABA (abscisic acid) response elements and MeJA (methyl jasmonate) response elements. The expression of FtNAC11 in different tissues of Tartary buckwheat had spatial specificity，and it was highly expressed in root and the seed of pre-grout stage (S1) and grout stage (S2). The expression levels of FtNAC11 decreased under ABA、MeJA、NaCl、SA(salicylic acid) stresses compared with the control. Overexpression of FtNAC11 significantly inhibited the germination rate and ratio of Arabidopsis seeds under the stresses of salt, drought and ABA. The results indicated that FtNAC11 has negative regulatory effects in response to the exogenous abiotic stresses, and provides a theoretical basis for further analyzing the stress response mechanism and regulatory function of FtNAC11 in buckwheat.
